Beer DNA sequencing

Requirements

The most important: 7.5µl DNA extracted as described in the DNA extraction protocol

Needed consumables

Needed material

Beer DNA sequencing

Library preparation

  1. Prepare tubes with 7.5µl extracted DNA and 2.5µl FRA
  2. Mix gently
  3. Put the tubes into a thermocycler: 1min at 30℃, 1min at 80℃
  4. Keep on ice until next step
  5. Attach an adapter (1µl of RAP) into the tube
  6. Mix gently with your fingers
  7. Incubate the reaction for 5 min at room temperature
  8. Store the library on ice until ready to load

Protocol: Software setup